Biochemical and Molecular Identification of Key Pathogens Causing Mastitis by Ro

Description: Biochemical and Molecular Identification of Key Pathogens Causing Mastitis by Robert Shavulimo Shivairo Estimated delivery 3-12 business days Format Paperback Condition Brand New Description Mastitis in dairy goats was investigated with the objective of establishing the effect of management and the identification of the key causative agents through common diagnostic procedures of somatic cell counts, bacteriological identification and, for the first time in goat mastitis, by use of Polymerase Chain Reaction. A cross-sectional survey, using a structured questionnaire was conducted in three agro-climatic regions of Coast, Nyanza and Rift Valley, with goat keeping clusters serving as sampling sub-units. The focus of the questions was on housing, feeding, labour, water sources, record keeping, socio-economic status of respondents and availability of extension service. California Mastitis Test (CMT) was done at the farm-level, Somatic Cell Counts (SCC) and bacterial isolation were done in the laboratory. Polymerase Chain Reaction (PCR) on the two key bacteria, Staphylococcus aureus and Escherichia coli were subsequently carried out on 16 randomly selected samples representative of the three dairy goat keeping agro-ecological zones. The management survey indicated that 56.9% of respondents were peasant farmers only 12% of respondents could afford hired labour. There was evidence of scarcity of quality water, with only 9% using water from rain catchment and the rest depending on wells, dams and rivers. There was no training package on dairy goats. The CMT scores for the two key organisms ranged between 2 and 3, making it a reliable test for udder infection. Somatic Cell Counts (SCC) was determined for 239 samples with scores ranging between 0.248 106 and 1.693 106 with a mean of 0.869 106. This study demonstrated significant SCC variations amongst the breeds, there was also significant variation in SCC scores for various locations. Other factors in the study were lactation length and parity all of which affected SCC scores. The bacterial isolation showed dominance of Staphylococcus aureus and Escherichia coli respectively. The two species of bacteria are significant indicators of the state of hygiene on the farms. The PCR identification of the two organisms showed that they were distributed in all three regions of study. There is a need for more intensive studies on the various diagnostic tools especially CMT, SCC, bacteriology and PCR to enable the development of quality standards in Kenya for goat milk which do not exist at the moment. Details ISBN 1502452731 ISBN-13 9781502452733 Title Biochemical and Molecular Identification of Key Pathogens Causing Mastitis Author Robert Shavulimo Shivairo Format Paperback Year 2014 Pages 114 Publisher Createspace Independent Publishing Platform GE_Item_ID:128266065; About Us Grand Eagle Retail is the ideal place for all your shopping needs! With fast shipping, low prices, friendly service and over 1,000,000 in stock items - you're bound to find what you want, at a price you'll love! Shipping & Delivery Times Shipping is FREE to any address in USA. Please view eBay estimated delivery times at the top of the listing. Deliveries are made by either USPS or Courier. We are unable to deliver faster than stated. International deliveries will take 1-6 weeks. NOTE: We are unable to offer combined shipping for multiple items purchased. This is because our items are shipped from different locations. Returns If you wish to return an item, please consult our Returns Policy as below: Please contact Customer Services and request "Return Authorisation" before you send your item back to us. Unauthorised returns will not be accepted. Returns must be postmarked within 4 business days of authorisation and must be in resellable condition. Returns are shipped at the customer's risk. We cannot take responsibility for items which are lost or damaged in transit. For purchases where a shipping charge was paid, there will be no refund of the original shipping charge. Additional Questions If you have any questions please feel free to Contact Us. Categories Baby Books Electronics Fashion Games Health & Beauty Home, Garden & Pets Movies Music Sports & Outdoors Toys

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End Time: 2025-01-21T12:52:17.000Z

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